Effect of phenol on ATPase activities in crude gill homogenates of rainbow trout (Salmo gairdneri Richardson)

by T. M Poston

Publisher: Dept. of Energy, [Office of Energy Technology], Pacific Northwest Laboratory, Publisher: for sale by the National Technical Information Service] in Richland, Wash, [Springfield, Va

Written in English
Published: Pages: 52 Downloads: 642
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Subjects:

  • Phenols,
  • Adenosine triphosphate -- Synthesis,
  • Rainbow trout

Edition Notes

StatementT. M. Poston
SeriesPNL ; 2697
ContributionsPacific Northwest Laboratory, United States. Dept. of Energy
The Physical Object
Paginationviii, 52 p. :
Number of Pages52
ID Numbers
Open LibraryOL14882265M

These cells are the main location of the gill Na +-K +-ATPase (3,6). Increased salinity results in the augmentation of Na + -K + -ATPase activity as well as morphological changes in CCs (3,7,8). The aim of the present study was to determine the effects of high salinity on gill Na + -K + -ATPase activity and. Phenol exposure kits can be obtained through Environmental Health and Safety for labs that use phenol. These kits have a five year shelf life. It includes a bucket with neoprene gloves in medium and large sizes (small upon request), decontamination instructions, 1 Liter of poly ethylene glycol MW solution, paper towels, bags for. The LibreTexts libraries are Powered by MindTouch ® and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. We also acknowledge previous National Science Foundation support under grant numbers , , and Phenol CAS # Division of Toxicology and Environmental Medicine September This Public Health Statement is the summary chapter from the Toxicological Profile for Phenol. It is one in a series of Public Health Statements about hazardous substances and their health effects. A shorter version, the ToxFAQsTM, is also available.

The Na + /K +-ATPase plays a major role in fish osmoregulation (Duarte et al., ), while SL seems to be involved with acid-base regulation in rainbow trout (Oncorhynchus mykiss) (Kakizawa et al., ) and correction of plasma osmotic balance in Mozambique tilapia (Oreochromis mossambicus) exposed to acidic freshwater (Furukawa et al., ). As a member, you'll also get unlimited access to o lessons in math, English, science, history, and more. Plus, get practice tests, quizzes, and personalized coaching to help you succeed. This Wildlife Toxicity Assessment summarizes available information on the likely effects of phenol on wildlife, stressing threshold doses for the onset of non-cancer effects, as described in reports of experimental studies of the compound. Surveying the threshold dosimetry of phenol may point to the. Coprinus cinereus peroxidase (CIP) was produced by Coprinus cinereus UAMH in a stirred fermentor; the fermentor broth was filtered, and used in phenol removal tests at room temperature. The impurities remaining in the crude enzyme solutions following filtration produced a beneficial effect on phenol removal from the buffered aqueous solution.

Overview. Phenol (above, carbolic acid) was isolated from coal tar in It served as a bacteriocide in the late 19th century. Phenol extraction first served to purify (deproteinize) carbohydrates (Westphal, et al. Z Naturforschung B 7, , ).It was subsequently adapted to "purify" nucleic acids (Kirby, Biochem J 64, , ).It also separates glycoproteins (Howe, et al, Meth. For freshwater life forms, acute toxicity levels of 5, to 7, ug/1 were reported, while for marine fish an acute value of nearly 3,, ug/1 was reported (both values for N-nitrosodiphenylamine). N-ni- trosodimethylamine has been shown to induce hepatocellular carcinoma."in rainbow trout. -IU   The effect of pH (in the pH range 3 to 11) in the presence of KI, KCl, and NaCl was also investigated. The concentration of phenol in the aqueous systems studied ranged from 10 to ppm. The temperature effect was also studied, and the resulting experimental equilibrium isotherms at 30, 40, and 55°C are well represented by Freundlich. biodegradation ofmonosubstituted phenols in phenol-adapted and control microcosms Test chemical and Vmax K. k1 (per microcosm (pg/liter (,g/liter) h) per h) m-Cresol Phenol 1 Phenol 2 Phenol 3 Initial community Lake Michie control Nutrient control m-Aminophenol Phenol 1 64 Phenol 2 66

Effect of phenol on ATPase activities in crude gill homogenates of rainbow trout (Salmo gairdneri Richardson) by T. M Poston Download PDF EPUB FB2

Get this from a library. Effect of phenol on ATPase activities in crude gill homogenates of rainbow trout (Salmo gairdneri Richardson). [T M Poston; Pacific Northwest Laboratory.; United States. Department of Energy.]. For both species the following Effect of phenol on ATPase activities in crude gill homogenates of rainbow trout book was adopted after optimising the conditions and solutions for incubating gill homogenates.

For each assay, 40 μl of gill homogenate in SEI medium was added to a glass test tube ( × 10 mm) containing μl of one of three different incubation media; +K medium, for total ATPase activity; −K/+ouabain (O, Sigma Chemical Co.) medium, which Cited by:   Measurement of gill Na + /K +-ATPase and H +-ATPase activity.

Gill tissue was scraped from filaments with a glass slide and homogenized on ice in SEID buffer (pHmmol l –1 sucrose, 10 mmol l –1 EDTA, 50 mmol l –1 imidazole, % sodium deoxycholate) using a ground glass homogenizer. Homogenates were centrifuged for 1 min (4°C) at g to remove filaments and Cited by: -ATPase, without effect on.

-ATPase activities of crude. cardiac homogenates from rainbow trout, crucian carp and rat. homogenates from rainbow trout, crucian carp and rat. The results are. K ’ -ATPase activity in crude gill homogenates of the longiawed mudsucker in this study were 5- to IO-fold higqer than reported by Doneen ( 1).

Effect of phenol at different salinities on the phosphomonoesterases and adenosine triphosphatase activity in the liver, muscle and brain of cichlid fishSarotherodon mossambicus (Peters) is studied. Results indicate a general decrease in the activity of all the three enzymes assayed.

The pattern of decrease shows a direct linear relationship with salinity and the toxicity is enhanced as. 1. The link between vitamin B 6 deficiency and sea water adaptation in rainbow trout was investigated. A plasma-hyperosmotic salinity (20%) exacerbates the effect of vitamin B 6 deficiency in rainbow trout.

Na/K-ATPase activity in the gill and kidney of the rainbow trout is not affected by vitamin B 6 deficiency.

The levels of Na+, K+ ATPase were measured in gills fromPlatichthys flesus adapted to seawater and freshwater using a variety of experimental techniques.

Na+, K+ ATPase was assayed directly in crude gill homogenates,3H-ouabain binding was determined in isolated, perfused gills and ouabainsensitive oxygen consumption measured in sliced gill filaments. INTRODUCTION. The gill is the major iono-regulatory organ in teleosts.

In the current model for active Na + uptake across tight epithelia, such as frog skin and the gills of freshwater (FW) teleosts, the driving force is thought to be set up by two membrane ion-pumps acting in series: an apical H +-ATPase and a basolateral Na +,K +-ATPase (Ehrenfeld and Garcia-Romeu, ; Avella and Bornancin.

A study of transfer rates of phenols from caustic to crude oil. Separation and Purification Technology, DOI: / Yue Wang, Fengge Zhai, Yasushi Hasebe, Hongmin Jia, Zhiqiang Zhang. A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode.

inhibitions were highest in the gill and brain homogenates for oligomycin-sensitive Mg2+ ATPase with pronounced effects (65%) after 50 days of exposure to ppm of Swanic. Fish exposed to lower concentrations showed an insignificant activation of Na -K ATPase and Mg2 ATPasc in the gills.

A similar study on in vivo responses of. The fate of radio-tagged hatchery-reared salmon smolts (Salmo salar L.) was investigated in the tailrace of a hydropower station in the Danish River Gudenaa during 2 years. Seventeen and 27 smolts were tagged and released in late May and during Aprilrespectively.

Out of the total of 44 smolts, only two were recorded to leave the river and enter the estuary. In both years of study. The energetic aspects of osmoregulation in several species of fish were examined, using an experimental approach on both a whole-animal and tissue level.

The first series of experiments examined the metabolic response of temperate and tropical fish species to acute and gradual salinity change, using whole-animal oxygen consumption rates and gill Na⁺,K⁺-ATPase activity as indicators of.

Proton ATPase activity in the crude homogenates of fish gill tissue. Inhibitor sensitivity and environmental and hormonal regulation. J Exp Biol –, Lin H, Pfeiffer DC, Vogl AW, Pan J, Randall DJ. Immunolocalization of H -ATPase in the gill epithelia of rainbow trout. J Exp Biol –, Lin YM, Chen CN.

Phenol is a colorless or white solid when it is pure; however, it is usually sold and used as a liquid. (1) The odor threshold for phenol is parts per million (ppm), with a strong very sweet odor reported.

(1,7) Phenol is very soluble in water and is quite flammable. (2) The chemical formula for phenol is. Copper nanoparticles (Cu-NPs) are components in numerous commercial products, but little is known about their potential hazard in the marine environments.

In this study the effects of Cu-NPs and soluble Cu on juvenile Epinephelus coioides were investigated. The fish were exposed in triplicate to control, 20 or µ g&#x;Cu&#x;L&#x;1 as either copper sulphate (CuSO. We identified five Na+/K+-ATPase α-isoforms in rainbow trout and characterized their expression pattern in gills following seawater transfer.

Three of these isoforms were closely related to other vertebrate α1 isoforms (designated α1a, α1b and α1c), one isoform was closely related to α2 isoforms (designated α2) and the fifth was closely related to α3 isoforms (designated α3).

ATPase, Mg2+-ATPase and Ca2+-ATPase) was observed. Moreover gill histopathological analysis on phenol exposure revealed severe lesions such as architectural loss, necrosis, desquamation of epithelial layer, hyperplasia and telangiectesis.

Significant changes observed on m-cresol exposure were. Phenol is lethal to fish even at relatively low levels, e.g. mg/L, depending on the temperature and state of maturity of rainbow trout (Brown et al. Phenolic compounds are also responsible for several biological effects, including antibiosis (Gonzalez et al.

), ovipositional deterrence (Girolami et al. ) and phytotoxicity. 1. Introduction. The contamination of aquatic ecosystems by metals is one of the main environmental issues today [].In the last decade, the advance of nanotechnology fueled fast growth of the nanotoxicology research and the need for information on potential hazards of this technology in aquatic environments [1, 2].Copper nanoparticles (Cu-NPs) are one of the most used nanomaterials due to.

Similar to studies concerning other salmonid presmolts (4, 21, 48), our Atlantic salmon had low gill Na +-K +-ATPase hydrolytic activities, with levels gradually increasing as smoltification advanced.

Enzyme activities rose substantially and, during the peak moment of smoltification, achieved a sevenfold increase in smolts compared with parr fish.

Proton ATPase activity in the crude homogenates of fish gill tissue. Inhibitor sensitivity and environmental and hormonal regulation. J Exp Biol –, ISI Google Scholar; 18 Lin H, Pfeiffer DC, Vogl AW, Pan J, Randall DJ.

Immunolocalization of H +-ATPase in the gill epithelia of rainbow trout. J Exp Biol –,   Introduction. Phenol (C 6 H 5 OH)is a monosubstituted aromatic hydrocarbon used to manufacture phenolic resins and plastics used in fertilizers, paints, textiles, adhesives, paper, and disinfectants.

Due to its anesthetic effects, phenol is also widely used in pharmaceutical products such as ointments, ear and nose drops, cold sore lotions, throat lozenges and sprays, and antiseptic lotions. Evidence for the presence of an electrogenic proton pump on the trout gill epithelium. J Exp Biol –, ISI Google Scholar; 80 Lin H, Randall DJ.

Proton-ATPase activity in crude homogenates of fish gill tissue: inhibitor sensitivity and environmental and hormonal regulation. J Exp Biol –, ISI Google Scholar.

In my thesis the cellular and subcellular distributions of various ion transport proteins were determined in the fish branchial epithelium using an immunological approach employing non-homologous antibodies.

The viabilities of current models of transepithelial ion transport in the gills of freshwater, and seawater fishes and an unusual amphibious air-breathing fish were tested. My thesis work. Phenol: health effects, incident management and toxicology Information on phenol (also known as carbolic acid and hydroxybenzene), for responding to chemical incidents.

Phenol is a colorless-to-white solid when pure; however, the commercial product, which contains some water, is a liquid. Phenol has a distinct odor that is sickeningly sweet and tarry. Most people begin to smell phenol in air at about 40 parts of phenol per billion parts of air (ppb), and begin to smell phenol in water at about 1�8 parts of phenol per million parts of water (ppm; 1 ppm is.

Phenol affects the nervous system and key organs, i.e. spleen, pancreas and kidneys (Manahan, ). Phenol is lethal to fish even at relatively low levels, e.g. mg/L, depending on the temperature and state of maturity of rainbow trout (Brown et al. table 1. Sources of phenol and other related aromatic compounds in wastewater.

Ans: The physical properties of phenol are: Phenols are usually colourless, toxic, corrosive, needle-shaped solids. They can soon liquify due to their highly hygroscopic nature. Phenol is less soluble in water but readily soluble in organic solvents.

Simplest phenols, because of hydrogen bonding have quite high boiling points. Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology.

We examined genomic an. Abstract -Phenol is a major pollutant in the wastewater because of its presence in the effluent of major processing and refining plants. It has severe effect on human being, both short term and long term. Various methods are used for removal of the phenol from wastewater such as adsorption, photodecomposition, volatilization.some water, is a liquid.

Phenol has a distinct odor that is sickeningly sweet and tarry. Most people begin to smell phenol in air at about 40 parts of phenol per billion parts of air (ppb), and begin to smell phenol in water at about parts of phenol per million parts of water (ppm; 1 .effects.

Phenol evaporates more slowly than water, and a moderate amount can form a solution with water. Phenol is used primarily in the production of phenolic resins and in the manufacture of nylon and other synthetic fibers. It is also used in slimicides (chemicals that kill bacteria.